Feng-Wei Lin is currently M.S. student in Institute of Medic Science and Technology at National Sun Yat-sen University, Taiwan. He received his B.S. degree in Materials Science and Engineering from Dayeh University, in 2014. His current research interests include the design and fabrication of nanoparticles and temperature-sensitive hydrogel for drug/gene delivery in cancer therapy.
Malignant brain tumor is a serious disease. Patients treated using surgical resection, radiotherapy and chemotherapy survive, on average, for 6-24 months post-treatment. To date, surgery still is the primary treatment for brain tumor, following with chemotherapy is necessary to prevent the tumor recurrence. However, many potentially effective diagnostic or therapeutic agents are prohibited to deliver into brain by blood-brain barrier (BBB), resulting in the failure of chemotherapy. To overcome the problems, we develop a mesoporous nanoparticles-incorporated temperature-sensitive hydrogel to slowly release drugs and gene in the brain tumor. Our preliminary data showed that hydrogel could be transferred to gel from solution phase while the temperature was raised higher to 32 ℃ then sustainably released the drugs over three weeks. This delivery system is expected to overcome the BBB, significantly reduce the side-effects and effectively prohibit the growth of residual tumor cells.
In the era of stressful life, different food habits associated with drug resistance, has lead to search for alternative health drinks. Morinda citrifolia, a plant from Rubiaceae family is available as a health tonic, also known as Noni juice in India. This juice has natural resources like Anthraquinone compounds extracted from root of Morinda citrifolia. This has reported anticancer properties through in vitro studies for antitumorigenic activity. Their potential target is p56lck, a protein essential for the development of T-cells and activity involved in the chemotactic responses of these cells. This protein is reported to play crucial role in breast cancer and colon cancer. Anthraquinones acts as a potent inhibitors of p56lck tyrosine kinase receptor in human cancer cells. Among anthraquinones derivatives, Damnacanthal was proved to induce cell growth arrest and trigger caspase activity in colorectal cancer cells and it is a potent inhibitor of p56lck tyrosine kinase activity. Till date, molecular level interactions between the anthraquinones and the receptor protein were not reported from in silico perspective. In the present study, we investigated the molecular interactions between Anthraquinones from the root of Noni with p56lck receptor using AutoDock’s Lamarckian genetic algorithm. Here we studied the binding site and behavior of 20 anthraquinones including Damnacanthal within the catalytic domain of p56lck receptor. Our results reports that apart from Damnacanthal, all other anthraquinones exhibits similar interactions in the active site of p56lck receptor and we proposed the molecular models of these anthraquinones with the receptor. Thus to conclude, the compounds derived from the root of Noni plant showed promising molecular specificity and interactions with the receptor involved in cancer cells.