5th World Congress on Cancer Therapy
Pathology Division, National Institute of Nutrition, India
Title: Effect of dietary fatty acids on development of carcinogen-induced breast cancer in female fisher (F344) rats
Biography: Surekha M V
Background and objectives: Objective of our study was to assess eff ect of dietary fatty acids (saturated vs unsaturated fatty acids vs transfats) in development of Dimethylbenzanthracene(DMBA) induced experimental mammary tumours in female fi sher rats. Methodology: Eighty weanling female fi sher (F344) rats were divided into fi ve groups of 16 each and fed with synthetic diets containing partially-hydrogenated vegetable oil / PHVO (transfat), palmolein (saturated fatty acids) , sunfl ower oil (n-6 PUFA), soyabean oil (α-linoleic acid) and sunfl ower + fi sh oil(LC n-3 PUFA) for 4 months aft er which 8 rats from each group were administered DMBA orally, once a week, for 4 weeks and continued on same diet for 8 months, while remaining 8 rats of each group were continued on respective diets. Results: Total serum SFA level was not aff ected by type of dietary fat, total MUFA level was highest in PHVO group, LC n-6 PUFA levels were not signifi cantly diff erent among groups and total LC n-3 PUFA levels were highest in sunfl ower oil + fi sh oil group. Number of tumours were least in PHVO and highest in n-6 PUFA group. Adenocarcinoma was observed as predominant tumour type and metastatic tumours, least common type, noted only in SFA diet group. Estrogen receptor positivity was mostly seen in n-3 PUFA group, progesterone receptor positivity in sunfl ower oil + fi sh oil group while PHVO group showed highest aromatase positivity. Conclusion: Dietary fatty acid composition to some extent refl ected in plasma phospholipid fatty acid composition. Transfats caused development of mammary tumours. N-3 PUFAs were associated with low and n-6 PUFAs with high tumour development.