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5th World Congress on Cancer Therapy

Atlanta, USA

H. Seda Vatansever

Celal Bayar University, Turkey

Title: Nanotubes and their functions in cancer

Biography

Biography: H. Seda Vatansever

Abstract

Since Rustom et al have shown formation of tunneling nanotubes (TNT) and published in Science journal in 2004 afterward this topic became crucial in forms of intercellular communication. Th ese formations have been called as tunneling nanotubes because of their diameter is around 50-200 nm and these nanotubes may easily move throug extracellular matrix to reach and communicate with other cells. TNTs are formated by F-Aktin polymerization in small tubes. On the other hand, in bigger tubes microtubul association has been observed during polymerization of F-Aktin. In time, there are some papers about tunneling nanotubes which have been published about normal and cancer cells. Especially, Ohno et al have shown that M-Sec is a central player of TNTs in Nature Cell Biology journal. Th is study presents that M-Sec protein also helps to formate protrusions of membrane as a marker of TNT. We hypothesize that M-Sec protein, central player of TNT formation, is overexpressed in these cancer cells which have overexpression of RalA. In this study, we aim to investigate roles, importance and diff erence of TNTs in primary and metastatic cancer cell lines. After WGA staining, there was TNT in metastatic colon (Colo-741) and breast (M4A4) cell lines. Nanotubes tunnelings were observed both the neighboring and cell colonies of metastatic colon and breast cancer cell lines have been identified. While RalA, RalB, RalAA/B and connexin-43 immunoreactivity were similar in both primer and metastatic colon and breast cancer cells, M-sec staining of colo-741 was found to be higher in the metastatic cell line. The results of Western blot analysis did M4A4 M-Sec in the metastatic group were also found to be higher than the Colo-741. Nanotubes tunnelings were observed especially in metastatic cancer cell lines (Colo-741, M4A4), and their presence in metastatic cell may be important for to propagation and survival of the cells can be achieved by the presence of TNTs and new approaches in the patient samples for treatment by testing the presence of TNT was concluded.