5th World Congress on Cancer Therapy
National Institute of Nutrition, India
Title: Sprague Dawley/NIN hairless mutant rat–A model to study carcinogenesis?
Biography: M. SatyaVani
Spontaneous mutations in rodents are valuable experimental models to study human disorders in biomedical research. Nude mutant rats are widely used in immunology, percutaneous drug absorption, wound healing, skin pharmacology and experimental carcinogenesis. Hypotrichosis (hairless) mutation was observed for the fi rst time in SD rat colony at National Centre for Laboratory Animal Sciences, NIN, India. Th e parents identifi ed were isolated and further propagation was carried out by selective breeding. Th e data on morphological features in each generation have been recorded. At present, the mutant is in 9th generation. Studies have been conducted to evaluate the new SD/NIN hairless mutant rat for biochemical, immunological and molecular characterization as well as histopathhological evaluation. Ninety days old (12 males and 12 females) animals both homozygous and heterozygous taken were housed using standard experimental conditions and observed for changes in ageing. Four females and two males developed spontaneous tumors. However, the dimensions as well as the weight were recorded and evaluated for histopathological analysis. Th e results of the histopathological evaluation showed that most of the tumors were fi bro -adenomas with predominant fi brous tissue which may progress to malignancy, if left untreated. Th e incidence of tumors was more in females than in males. As these mutant rats are hairless, depilation (time consuming) is not required and therefore, the tumors can easily be identifi ed. Based on the above features it is presumed that mutant model may be used to study carcinogenesis and tumor therapy in future.