5th World Congress on Cancer Therapy
National Autonomous University of Mexico, Ciudad Universitaria, Mexico
Title: The presence of aflatoxin B1-FAPY adduct and Human Papilloma Virus in cervical smears from cancer patients in Mexico
Biography: Magda Carvajal
Many natural foods (cereals, spices, oilseeds, etc.) are contaminated by the fungi Aspergillus spp. that produce the carcinogens called afl atoxins, they are inevitable risks for all humans who are consuming them daily, also in processed foods. Th ese AF are activated in the human liver where the carcinogenic biomarker afl atoxin B1-formamidopyrimidine 2,3-dihydro-2-(Nformyl)- 20, 50, 60-triamino-40-40-oxy-N-pyrimidyl-3-hydroxy-AFB1 called AFB1-FAPY adduct is formed, and it is stored in the DNA for years. In the present research this AFB1-FAPY adduct was quantifi ed as well as Human Papilloma Virus (HPV) types 16 and 18 identifi ed from DNA cervical scrapes from 40 women with cervical cancer (CC) and 14 healthy women as controls. Th e relationship between the AFB1-FAPY adduct and HPV types 16 and 18 was determined. Competitive inhibitory indirect ELISA was validated with 94% inhibition to quantify the AFB1-FAPY adducts in picograms per milligram of DNA (limit of detection= 0.1 pg/mg, and limit of quantifi cation= 10 pg/mg), polymerase chain reaction and DNA sequencing to identify HPV types. Th e average concentration of AFB1-FAPY adducts/mg DNA in the CC cases was 1025 pg, 1420 pg with HPV16 and 630 pg sharing HPV18 (p= 0.03). In comparison, healthy controls had 2.6 pg/mg DNA, a statistically signifi cant diff erence (p≤ 0.00006). Th e presence of AFB1-FAPY adduct increased six-fold the risk for CC between cases and controls, the odds ratio was 6.1 (95% CI= 1.4–25.4). Th ere was a close relationship between the AFB1-FAPY adducts and HPV16 in CC samples. Th e conclusion is that cervical cancer is originated by both carcinogens AFB1-FAPY adduct and HPV.