5th World Congress on Cancer Therapy
ESC Taras Shevchenko National University, Ukraine
Title: UV light analisys of anticancer drug NSC 631570 in urine samples sorts healthy donors and prostate adenocarcinoma patients. Could the different drug up-take used to establish free-diagnosis cancer patients?
Biography: Larysa M Skivska
Introduction: Th e mixture of Alkaloids (NSC-631570) have been showed an anticancer activities against diff erent types of solid tumor. In particular this activities in combination with gemcitabine signifi cantly increased the median survival of advanced PDAC patients with respect to gemcitabine alone (10.4 vs 5.2 months; p<0.001). Furthermore, other studies showed an anticancer eff ect in prostate cancer patients also. Indeed, the cytotoxic eff ects of NSC-631570 was evident in preclinical studies in prostate cancer cell line (PC-3). In particular, using the auto-fl uorescence properties of this Drug (by UV light excitation) some author showed a diff erent up-take of drug in PDAC cells with respect to normal epithelial cells of pancreas (HNPE). Th e data of literature seem to indicate that this mixture of alkaloids had selective in cancer cell lines derived from diff erent tumor types, but not in normal cell lines. Aim: To investigate the concentration NSC 631570 in urine samples of prostate cancer patients using UV light approach to confi rm diff erent up-take of alkaloids mixture in both normal and prostate cancer patients. Materials & Methods: We studied opctical characteristics in urine samples (before and aft er the sublingual administration of the drug)using two diff erent absorption spectrums: UV light and fl uorescence at λ = 540 nm. We observed the variation of both intensity at 0,2,4 and 6 hours aft er administration. Urine samples from 2 prostate cancer patients (PC1 and PC2) and 3 healthy controls (HCs) were used. Results: Th e UV light absorption spectrum of urine HCsaft er NSC 631570 coincided almost completely with that of the drug. PC1 showed a similar absorption spectrum similar to NSC 631570 aft er 4 hours sublingual administration only. While, absorption spectrum ofPC2 was completely diff erent during the entire observation period compared with those observed in HCs (p<0.05). Urine fl uorescence PC1 increased in both PC1 and PC2 aft er the drug administration, till the end of the observation period nonlinearly. However, 6 hours aft er administration of the drug a moderate decrease of the fl uorescence intensity has been registered. Conclusion: Comparative analyses of urine optical characteristics of both healthy donors and prostate adenocarcinoma patients have been shown that absorption spectra under the UV light aft er sublingual administration of the drug NSC 631570,makes the diff erences betweenprostate adenocarcinoma and healthy patients. Probably, these data indirectly indicate diff erences concerning dynamics clearance of drug in diff erent subjects. Comparative study of urine fl uorescence intensity of healthy donors and patients with cancer pathology at λ = 540 before and aft er sublingual administration of the drug NSC 631570 in dynamics proved to be uninformative. In conclusion this method could be used to test whether non clinically pathologic subject might be aff ected by cancer.