Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 14th Asia Pacific Oncologists Annual Meeting Melbourne, Australia.

Day 1 :

Conference Series Asia Pacific Oncologists 2017 International Conference Keynote Speaker Ayten Demir photo
Biography:

Ayten Demir has graduated from Cumhuriyet University School of Nursing. She has completed her Master’s degree in Institute of Health Sciences and also completed Doctorate degree. She is working as Professor at Ankara University. She has had various studies in national and international journals and book chapters related to her field.

Abstract:

The study aimed to put forth an easy-to-use, non-side effect, vegetable origin and alternative oral care solution for wound healing via the root extracts of Ononis spinosa leiosperma (L) which is an alternative anti-inflammatory and anti-oxidant medicine on sore healing. A total of 24 male Wistar Albino rats in this animal research were randomly grouped into three groups, each with eight rats. Group 1 (Control group), Group 2 (Chlorhexidine group) and Group 3 (1% Ononis spinosa group) were given 90 mg/kg Ketamine, 10 mg/kg Xylazine anesthesia intramuscularly on day 0. Following the anesthesia, 5 mm radius, 1 mm depth wounds were induced on the rats' cheek mucosa by standard biopsy techniques and punch. For 10 days, Group 1 was observed and the other groups were treated the following mouth care: Group 2 (Chlorhexidine group) 2×1 cc Chlorhexidine, Group 3 (1% Ononis spinosa group) 2×1 cc 1% Ononis spinosa extract. After these applications, macroscopic and histopathological analyses were carried out on tissue samples obtained from local area. According to the macroscopic and histopathologic analysis, it was detected that 1% Ononis spinosa roots extract accelerated the healing process and reduced complications much more than the control and Chlorhexidine group. Results showed us that 1% Ononis spinosa extract increased wound healing activity remarkable with a 99.3% contraction rate in wounds of tongue dorsum and 87.7% in buccal mucosa at the 10th day. More experimental and clinical studies in larger populations are needed to prove and confirm the efficiency of the study.

Keynote Forum

Qiong Ma

Fourth Military Medical University, China

Keynote: SKA1: A therapeutic target for chemo-resistance in human osteosarcoma

Time : 9:30-10:15

Conference Series Asia Pacific Oncologists 2017 International Conference Keynote Speaker Qiong Ma photo
Biography:

Qiong Ma has her expertise and interest in scientific study on human osteosarcoma, especially about the proliferation, invasion and metastases of tumor cells. She has studied tumor cells under hypoxic conditions.

Abstract:

The combination of aggressive surgery and neoadjuvant chemotherapy is also the major treatment for human osteosarcoma nowadays. However, the issue of chemo-resistance development has sustained and poses a great challenge. Researchers have reported that hypoxia may lead to drug resistance in many kinds of solid tumors. The mechanism is not very clear yet. The purpose of this study is to explore how hypoxia leading to chemo-resistance in osteosarcoma cells. We scanned normoxia and hypoxia cultured osteosarcoma cells in silico in three replicates to find the differential expressed gene under hypoxic condition, and this gene was overexpressed by lenti-virus vector, then real-time PCR and western were utilized to detect the expression of three drug resistance related genes ABCB1, ABCC2, and GSTP1. Cell Counting Kit-8 (CCK8) assay was performed to evaluate the proliferation of cells after lenti-virus transfected. 545 differentially expressed genes were identified based on the microarray analysis. Attention was focused on the SKA1 gene as a possible downstream target of hypoxia by means of bioinformatics. SKA1 overexpression reduced the expression of three multidrug resistant genes ABCB1, ABCC2 and GSTP1. Also, we demonstrated that SKA1 overexpression enhanced the sensitivity of two chemotherapeutic drugs used for osteosarcoma patients. Our study made an attempt to identify the downstream target genes that are altered in the hypoxia-cultured osteosarcoma cell line by microarray analysis. We demonstrated that the expression of SKA1 was significantly decreased in a hypoxic environment. And SKA1 may function as a chemosensitizer in osteosarcoma. A strategy to enhance its expression may prove to be beneficial for the treatment of osteosarcoma.

Session Introduction

Qiong Ma

Fourth Military Medical University, China

Title: A therapeutic target for chemo-resistance in human osteosarcoma
Speaker
Biography:

Qiong Ma has her expertise and interest in scientific study on human osteosarcoma, especially about the proliferation, invasion and metastases of tumor cells. She has studied tumor cells under hypoxic conditions.

Abstract:

The combination of aggressive surgery and neoadjuvant chemotherapy is also the major treatment for human osteosarcoma nowadays. However, the issue of chemo-resistance development has sustained and poses a great challenge. Researchers have reported that hypoxia may lead to drug resistance in many kinds of solid tumors. The mechanism is not very clear yet. The purpose of this study is to explore how hypoxia leading to chemo-resistance in osteosarcoma cells. We scanned normoxia and hypoxia cultured osteosarcoma cells in silico in three replicates to find the differential expressed gene under hypoxic condition, and this gene was overexpressed by lenti-virus vector, then real-time PCR and western were utilized to detect the expression of three drug resistance related genes ABCB1, ABCC2, and GSTP1. Cell Counting Kit-8 (CCK8) assay was performed to evaluate the proliferation of cells after lenti-virus transfected. 545 differentially expressed genes were identified based on the microarray analysis. Attention was focused on the SKA1 gene as a possible downstream target of hypoxia by means of bioinformatics. SKA1 overexpression reduced the expression of three multidrug resistant genes ABCB1, ABCC2 and GSTP1. Also, we demonstrated that SKA1 overexpression enhanced the sensitivity of two chemotherapeutic drugs used for osteosarcoma patients. Our study made an attempt to identify the downstream target genes that are altered in the hypoxia-cultured osteosarcoma cell line by microarray analysis. We demonstrated that the expression of SKA1 was significantly decreased in a hypoxic environment. And SKA1 may function as a chemosensitizer in osteosarcoma. A strategy to enhance its expression may prove to be beneficial for the treatment of osteosarcoma.

Speaker
Biography:

Bo Yuan has completed his PhD from Tokyo University of Pharmacy & Life Sciences and had researched in University of California, San Francisco as a Visiting Assistant Professor. He is an Assistant Professor in TUPLS. His research interests focus on the novel antitumor effect of clinically used antitumor drugs in combination with naturally occurring phytochemicals in terms of sensitization of cancer cells to drugs resulting in dosage reduction for clinical application. He has published more than 40 papers in reputed journals.

Abstract:

To provide novel insight into the development of new therapeutic strategies to combat breast cancer using trivalent arsenic (AsIII)-based combination therapy, the cytotoxicity of a combination of AsIII and tetrandrine (Tetra), a Chinese plant-derived alkaloid, was investigated in the human breast cancer cell line MCF-7. Cytotoxicity was evaluated using cell viability, colony formation, wound healing, lactate dehydrogenase leakage and cell cycle assay. Alterations of genes associated with cell proliferation and death were analyzed using real-time PCR and western blot. Intracellular arsenic accumulation (As[I]) was also determined. Tetra significantly enhanced the cytotoxicity of AsIII against MCF-7 cells in a synergistic manner. The combined treatment upregulated the expression level of FOXO3a, and subsequently resulted in a concomitant increase in the expression levels of p21, p27 and decrease of cyclin D1, which occurred in parallel with G0/G1 phase arrest. Autophagy induction was also observed in the combination treatment. Importantly, combining AsIII with Tetra exhibited a synergistic inhibitory effect on the expression level of survivin. Furthermore, enhanced As[I] along with synergistic cytotoxicity was observed in MCF-7 cells treated with AsIII combined with Tetra or Ko134, an inhibitor of breast cancer resistance protein (BCRP), suggesting that Tetra or the BCRP inhibitor probably intervened in the occurrence of resistance to arsenic therapy by enhancing the As[I] via modulation of multidrug efflux transporters. These results may provide a rational molecular basis for the combination regimen of AsIII plus Tetra, facilitating the development of AsIII-based anticancer strategies and combination therapies for patients with solid tumors, especially breast cancer.

Speaker
Biography:

Amar Ranjan is an MD in Pathology, presently working as an Assistant Professor in Lab Oncology in the top most Cancer Institute of India. He has keen interest in Hemato-oncology. He actively participates in oral and poster presentations at international and national levels. He has experience of working on dermatopathology, post-mortem pathology and blood banking.

Abstract:

Thrombopoietin (TPO) is a protein that is encoded by the TPO gene. It regulates the production of platelets. It is believed that plasma level of TPO is regulated by its binding to platelets and megakaryocytes. A prospective study was conducted comprising of 72 cases (32 female, 40 male) of gastrointestinal cancer, which were undergone surgery in the year 2016. It included cancer of esophagus, stomach, colon and ano-rectum. Three serial whole blood samples were taken from single patient, one preoperatively, 2nd and 3rd postoperatively on day 3 and day 5. Serum samples were stored at-80 °C. Samples were tested for TPO and PCT by ELISA Technique. Statistical analysis was done. Day 3 after surgery, patients (n=72) showed a significant thrombocytopenia followed by a reactive thrombocytosis on Day 5. Platelet recovery was preceded by a significant rise in TPO (from 162.4±118.8 pg/ml at baseline to 355.3±304.4 pg/ml at 72 hours, P<0.0001), which in turn was preceded by a marked increase in PCT (from 141.7±406.4 pg/ml at baseline to 659.6±1087.0 pg/ml at 72 hours, P<0.0001). The rise of both PCT and TPO was significantly higher in all patients at an interval of 3-4 days. No correlation was found between the post-operative decrease in platelet mass and changes in either the TPO or PCT levels. Considering the change of parameters from day 3 to 5, there was rise in platelets and decrease in TPO and PCT. These changes were not found statistically significant. But statistically significant changes were noticed from day 1 to day 5 similar to day 1 to day 3. Findings suggest that circulating TPO levels, besides being controlled by changes in platelet mass, are also influenced by certain cytokines involved in oncogenesis and inflammatory process. Studies suggest that it is influenced by IL-6. It has shown activities like acute phase reactants e.g., C-reactive protein.

Speaker
Biography:

Kirsty Hamilton is a Neurosurgical Trainee, currently practicing at the Princess Alexandra Hospital, Brisbane. Her research work deals with intra-medullary spinal cord tumors, which was undertaken to address a knowledge gap in the literature for intramedullary tumor treatment strategies

Abstract:

The true impact of surgical resection and adjuvant therapies on survival in intramedullary ependymoma and astrocytoma is largely unknown. Searching of Medline, Embase and Clinicaltrials.gov databases were performed. Multivariate analyses were performed for overall survival (OS) and progression-free survival (PFS) data sets. This was achieved through a combination of Monte-Carlo methods and maximum likelihood estimation. 57 articles yielded results for 3022 patients. Gross-total resection (GTR) reduces mortality in both ependymoma and astrocytoma by a factor of 5.1. An interaction was identified between tumor grade and radiotherapy, such that for low-grade tumors, radiation treatment increased the risk of mortality 5.2 times, while for high-grade tumors radiotherapy decreased mortality by a factor of 1.9. High-grade tumors were associated with a 12 times risk of death over low-grade tumors. Adult patients were more likely to die from their disease compared with pediatric patients by a factor of 1.6. Regarding PFS, radiation treatment increased the rate of morbidity 1.9 times for both pathologies. Gender did not influence survival. 79% of patients demonstrated stable or improved functional neurological outcomes six months post-operatively. GTR improves OS in all tumor grades. Adjuvant radiation improves OS only in the presence of high-grade histology. Advancing age and high-grade histology are negative prognostic indicators. Gender does not influence survival.

Speaker
Biography:

Larysa Skivka has completed her PhD from RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine and Postdoctoral studies from Taras Shevchenko National University of Kyiv. Currently she is a Professor of the Educational and Scientific Centre, Institute of Biology and Medicine of Taras Shevchenko National University of Kyiv, Ukraine, Head of the Department of Microbiology and Immunology. Her area of scientific activity includes immunomodulation as a component of adjuvant cancer therapy, functional polarization of phagocytes in the pathogenesis of inflammatory diseases.

Abstract:

Glioblastoma (GB) is one of the most devastating and fatal tumors. Therapeutic approaches targeting tumor cells have failed. GB is heavily infiltrated with myeloid cells that are collectively referred to as glioma-associated microglia/macrophages or GAM. GAM acquires the alternative, pro-invasive phenotype and creates favorable conditions for disease progression. GAM re-education seems to be an attractive therapeutic approach to GM treatment. NSC631570 is an anticancer agent that influences phagocyte migration and functional polarization. This study was aimed to investigate the effect of NSC631570 on C6 glioma growth in rats and microglia metabolic profile in vitro and in vivo. For tumor-associated hypoxia induction experiments, the rat microglial cells were treated under either normoxia (21% O2) or hypoxia (3% O2) for 48 hours in vitro. Intracranial drug delivery device was developed for the local treatment of glioma-bearing rats with NSC631570. Tumor-bearing animals received seven intracranial injections of the drug at 3 days interval starting from the second day after C6 cell transplantation. NSC631570 re-polarized hypoxic microglia to pro-inflammatory metabolic profile in vitro. The treatment of tumor-bearing rats with NSC631570 was associated with prolongation of their life by 18%. This effect was accompanied by the increase in the number of phagocytizing CD14+ cells in the microglia fraction without the alteration in their endocytosis intensity. The frequency of CD206+ cells was moderately increased in the fraction of both CD14+ and CD14-microglial cells. Oxidative metabolism of GAM was moderately down-regulated by the drug. Intracranial introduction of the preparation was associated with the sharp increase of NO generation by microglial cells. Locally introduced NSC631570 can re-educate GAM. This repolarizing effect is associated with moderate tumor growth inhibition and might be considered as an important part of the mechanism of tumor-inhibiting action of the drug.

Naila Amin Nitu

Ministry of Health and Family Welfare, Bangladesh

Title: Modeling the cost-effectiveness of using digital breast tomosynthesis in breast screening program

Time : 14:00-14:30

Speaker
Biography:

Naila Amin Nitu is a Bangladeshi Physician practising Gynaecology and Public Health. She has 14 years of experience in maternal health, community health services, clinical service delivery, training and research. She currently serves as the Deputy Director of the Health Economics Unit of Ministry of Health and Family Welfare of Bangladesh Government. She engages in conducting policy-oriented research on health economics and works for advancing the Universal Health Coverage for Bangladeshi population and maintains collaboration with the donor organizations. She has spent more than 10 years to work for the underprivileged women to ensure their better health. She has achieved Fellowship of College of Physicians and Surgeons in 2009 from Bangladesh and obtained an Australian Award Scholarship in 2015 to pursue Master of Public Health with specialisation in Health Economics and Economic Evaluation at the University of Melbourne where she graduated in 2016.

Abstract:

Background: In Australia, breast cancer was the second most common cause of cancer death in 2011. Currently, all Australian women aged 50-69 years are invited to attend biennially in population-based breast screening program with Digital Mammography (2D). But 2D screening fails to detect at least 15-30% of breast cancers. However, with Digital Breast Tomosynthesis (3D), more cancers would be expected to be diagnosed earlier compared to 2D screening.

Objective: The study aims to compare the cost-effectiveness of biennial screening with 3D with biennial screening with 2D for women aged 50-69 years from the healthcare system perspective.

Method: A Markov model was constructed to capture the costs and effectiveness of screening and diagnostic pathway of both screening programs including the stage-specific treatment of breast cancer. All estimates for model input were derived from published articles. This model was created with a time horizon of 35 years and 2 weeks cycle length has been created. One-way and probabilistic sensitivity analysis was conducted.

Results: The base-case analysis estimated that the discounted incremental cost-effectiveness ratio is $40,923/QALY gained for 3D screening compared to 2D screening. 3D screening reduces the chance of biopsy and ultrasonography and increases the cancer detection at an early stage compared to 2D. Our analysis indicates that women spend comparatively more time in better health states with 3D screening compared to 2D. However, sensitivity analysis shows that considerable amount of uncertainty exists around these estimates.

Conclusion: Biennial 3D screening seems to be cost-effective compared to 2D screening for women aged 50-69 years. These results could be a strong basis to consider the implementation 3D screening in the population-based breast screening program. However, further research is warranted with better transition probability parameters of the effectiveness of 3D screening with clinical trials which would give more precise estimates of the cost-effectiveness analysis.

Biography:

Kwong Soke Chee is currently a PhD student at University of Malaya, Malaysia. Her research interest includes molecular biology and lipidomics. In specific, her project focuses on the function of FABP7 in TNBC. Besides doing bench work in the laboratory, she also has experience in recruiting patients for breast cancer cohort. In year 2015, she was selected to participate in Novartis International Biocamp in Basel, Switzerland to gain insights into research and business environment in pharmaceutical industry.

Abstract:

Triple negative breast cancer (TNBC) is the most aggressive subtype which contributes to approximately 10% of breast cancer cases. The absence of ER-, PR- and HER2 receptors in TNBC leaves this subtype with no targeted therapy. Recent studies showed that FABP7 is shown to significantly overexpress in TNBC tissues compared to other subtypes. FABPs are known to be lipid chaperones and they can affect lipid metabolism. To date, the evidence on its prognostic role in TNBC is contrasting. Liu et al. (2012) shows that FABP7 expression correlates with lower overall and recurrence-free survival. In contrast, two other studies by Alshareeda, et al. (2012) and Zhang, et al. (2010) demonstrates that FABP7-positive basal tumors are associated with better prognosis. Hence, we aim to investigate the function of FABP7 in TNBC through in vitro models. Despite the excessive FABP7 expression in TNBC tissue, FABP7 protein was not detected in TNBC cell lines (HS578T and MDA-MB-231). However, chronic hypoxia increased FABP7 mRNA expression in these cell lines. It indicates that FABP7 might only be important in hypoxic conditions. As FABP7 was not naturally expressed in the TNBC cell lines used in our study, we generated FABP7-transduced TNBC cell lines with lentivirus particles. MTT assay showed that FABP7 caused reduced cell viability in HS578T but not MDA-MB-231 cells under hypoxic condition. This study shows that FABP7 can cause cell death in HS578T cells under hypoxic condition but not in the more aggressive MDA-MB-231 cells. More research on FABP7 in TNBC is warranted as it could serve as a potential molecular target for TNBC.

Biography:

Eleane Ayou has completed her Bachelor’s degree in General Medicine and Surgery from Duhok University, College of Medicine and currently she is a Trainee toward specialty in Obstetrics and Gynecology. She is one of the Senior Registrars at both Duhok Maternity Hospital and Azadi Teaching Hospital in the city of Duhok, Iraq.

Abstract:

Background: Although Gestational trophoblastic disease (GTD) is mostly a benign condition, malignant transformation may occur. It is the most curable disease among all the gynecological malignancies especially when early diagnosis is made. Despite that, there is paucity of local data regarding the burden of this condition, its management and outcome.

Objectives: The study aims to assess the data for the prevalence, treatment protocols and outcome of GTD in cases admitted to Azadi Teaching Hospital, Duhok, Iraq.

Methods: A retrospective and prospective analysis of cases documented during the period from February 2011 to July 2017. Ninety-six (96) cases were included. Retrospective data were retrieved from patients’ medical records and GTD special registry while prospective data were recorded in patients’ record at Gynecology Clinic and were updated with each visit. Human chorionic gonadotropin hormone level was the main investigation we based on for diagnosis and follow up.

Results: Seventy (72.9%) cases were multigravida, forty-three (44.8%) were between the age 21-30 years. All patients had vaginal bleeding at presentation. Only five (5.2%) cases had extra uterine metastasis, two (2%) patients had history of previous GTD, four (4.2%) patients ended with hysterectomy 23 (23.9%). Patients were solely treated with dilatation and curettage without need for any chemotherapy, 62 (64.8%) patients were treated successfully with single agent chemotherapy while 11 (11.6%) patients needed multi-agent’s chemotherapy.

Conclusion: No patient died from GTD during this period. Among patients who needed chemotherapy, most of our cases had good response to single agent chemotherapy.