15th Asia Pacific Oncologists Annual Meeting
Hashemite University, Jordan
Title: Biochemical and XRCC1 gene polymorphisms as risk factor for lung cancer among Jordanian patients
Biography: Manar Atoum
Lung cancer is the leading cause of cancer-related deaths worldwide. Malondialdehyde (MDA) is one of reactive species that plays a role in increasing DNA damage. Superoxide dismutase (SOD) is an important antioxidant enzyme that protect against ROS injury in lung. X-ray cross complement gene (XRCC1) is a DNA repairing gene that has an important role in repairing DNA alterations. Arg399Gln polymorphism in XRCC1 may affects the risk of lung cancer. So, the aim of this study is to determine the association between MDA, Cu-Zn Superoxide dismutase serum level and Arg399Gln polymorphism among Jordanian lung cancer patients.
This study conducted in Al-Basheer hospital between (2015 and 2016) and enrolled thirty five lung cancer patients and forty healthy subjects. MDA serum level was measured using colorimetric assay and the serum level of (Cu-Zn SOD) was determined using ELISA assay. DNA samples were amplified by PCR, genotyped using MspI restriction enzyme digestion and electrophoresed on agarose gel.
MDA serum level was significantly higher among lung cancer patients (3.34 ±0.6 nmol/mL) compared to control (2.72 ±0.49 nmol/mL) and associated with around ten folds increased risk for lung cancer (OR 2.54). (Cu-Zn SOD) serum level was significantly lower in lung cancer patients (50.03 ±9.6 ng/mL) compared to control (67.26 ±13.2 ng/mL) and associated with more than three folds increased risk for lung cancer (OR 3.85).
Significant differences were found in genotypic and allelic distribution of XRCC1 gene Arg399Gln polymorphism between lung cancer patients and control. GA, AA genotypes and A allele were more frequent in lung cancer patients (52.8%, 15.1%, 41.5%) compared to control (38.8%, 2%, 21.4%) and significantly associated with increase lung cancer risk (GA; OR 2.51), (AA; OR 13.65),(A; OR 2.60). No significant association was found between serum levels of MDA, (Cu-Zn SOD) and XRCC1 Arg399Gln polymorphism among lung cancer patients and control group.
Conclusion: high serum levels of MDA and low serum levels of (Cu-Zn SOD) are associated with increased lung cancer risk among Jordanian lung cancer patients. AA, GA genotypes and A allele of the XRCC1 Arg399Gln are also associated with increased lung cancer risk.