8th Euro Global Summit on Cancer Therapy
Western University, Canada
Title: Systematic Identification and Characterization of the Methylproteome for Cancer Intervention
Biography: Shawn S.C. Li
Methylation of Lys and Arg residues has emerged as a prevalent post-translational modifi cation (PTM) occurring on numerous non-histone proteins, drastically extending its role beyond the known histone code. We have developed an approach that combines mass spectrometry with peptide arrays and bioinformatics to systematically identify protein methylation and quantify dynamic changes in the methylome associated with tumorigenesis or drug resistance. Our studies not only led to the identifi cation of numerous novel methylation sites, but also generated new insights into how protein methylation regulates cellular functions such as DNA damage repair, apoptosis and drug resistance. With recent advances in mass spectrometry, the stage is now set to decode the methyl proteome and to elucidate, systematically, the mechanisms of interplay between histone and non-histone methylation and between methylation and other types of PTM for cancer diagnosis and intervention.