Raju N Gacche
Swami Ramanand Teerth Marathwada University, India
Title: Prosopis juliflora leaves extract induces apoptosis in the breast cancer cells
Biography
Biography: Raju N Gacche
Abstract
In recent years plant originated drugs/formulations are extensively prescribed as complementary therapy for treating various human ailments including cancer. On the eve of evolving multi drug resistance in treating cancer, medicinal plants have remained a significant hope for identification of novel anti-cancer leads. Prosopis juliflora (Sw.), DC is a xerophytic, desert plant species which grows in an extreme environment. In the present study, the methanol leaves extract of P. juliflora has been assessed for its anti-breast activity against MDA-MB-231: A triple negative hormone independent and ER+, PR+ hormone dependent MCF-7 tumorigenic breast cell lines, along with using a control of HaCaT: A non-tumorigenic human keratinocyte cell line. Series of experiments like MTT cell proliferation assay, Annexin V (apoptosis) detection assay, cell cycle analysis, ROS detection assay, colony formation assay (Clonogenic assay) and soft agar assay were performed to address the possible mechanism of action of P. juliflora leaf extract on selected breast cancer cells. The results of the various assays indicates that the methanol leaves extract of P. juliflora possess promising anti-cancer activity against MDA-MB-231 cell line at 25 µg/mL. However, it did demonstrate effectiveness against MCF-7 breast cancer cell line and a non-tumorigenic HaCaT keratinocytes. Arresting the cell growth by inducing apoptosis, seems to be a possible mechanism of action of P. juliflora.