Rama Swamy Nanna
Kakatiya University, India
Title: Determination of anticancer activity of aqueous leaf extracts of Senna alata using MTT assay
Biography
Biography: Rama Swamy Nanna
Abstract
Senna alata is an important ethnomedicinal plant belonging to Fabaceae family. The plant is widely used in folk medicine for its laxative and antifungal properties. The extracts of the plant are also known to possess a wide range of medicinal properties like antimicrobial, antioxidant, anti tumor, antipyretic and analgesic properties. In the present investigation an attempt has been made to study the anticancer activity attributed to the aqueous leaf extract of the species using MTT assay. In vitro anticancer activity of aqueous leaf extracts of Senna alata was carried out on human Breast Cancer (MCF-7) cell line using MTT assay. After treating the MCF-7 cells with the standard and test drugs and subsequent incubations, OD of Tamoxifen (STD) was compared to that of the test extract at 492 nm and percentage of inhibition of cell proliferation was calculated. The results of the present study showed that an increase in the inhibition of cell proliferation was concentration dependent and even at lower concentrations the test extracts effectively regulated cell proliferation. As the concentration of the test extract increased from 6.25-200 µg/ml, an increase in the percentage of cell inhibition was observed and at highest concentration (200 µg per ml) of the leaf extract the maximum percentage of cell inhibition (99.92%) was observed. The leaf extract of S. alata can be used to cure the breast cancer.